Article Text


Value of surgical resection and timing of therapy in patients with pancreatic cancer at high risk for positive margins
  1. Anna Torgeson1,
  2. Ignacio Garrido-Laguna2,
  3. Randa Tao1,
  4. George M Cannon3,
  5. Courtney L Scaife4,
  6. Shane Lloyd1
  1. 1 Department of Radiation Oncology, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA
  2. 2 Department of Internal Medicine, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA
  3. 3 Department of Radiation Oncology, Intermountain Medical Center, Murray, Utah, USA
  4. 4 Department of Surgery, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA
  1. Correspondence to Dr Shane Lloyd, Department of Radiation Oncology, Huntsman Cancer Hospital, Salt Lake City, UT 84112, USA; Shane.Lloyd{at}


Background Surgical resection remains the best chance at long-term survival in pancreatic cancer, though margin-positive resections are associated with diminished survival. We examined the effect of margin-positive resection on survival, as well as the role and timing of additional therapies through the National Cancer Database (NCDB).

Patients and methods Patients with stage IIA–III pancreatic adenocarcinoma diagnosed from 2004 to 2013 were identified in NCDB. Survival was compared using univariate and multivariate Cox proportional hazards modelling for patients who underwent surgery with negative (R0), microscopically positive (R1) and macroscopically positive (R2) margins or non-surgical treatment. We further analysed patients by margin status, timing of additional therapy (neoadjuvant therapy (NAT) vs adjuvant therapy (AT) vs none) and clinical stage.

Results We analysed 44 852 patients. Median survival (MS) for patients who did not undergo surgery was 10.3 months, compared with 19.7 months for R0 (P<0.001), 14.3 months for R1 (P<0.001) and 9.8 months (P=0.07) for R2 resections. NAT (MS 23.2 months) was associated with improved survival compared with AT (MS 21.5 months) in negative-margin patients and equivalent (MS 17.6 months) to AT (MS 16.8 months) in positive-margin patients. Survival for stage III NAT positive-margin patients (MS 19.8 months) was equivalent to AT after negative margins (MS 18.4 months, P=1.00). Improved R0 rates were seen with NAT (88% vs 81%, P<0.001), especially in stage III patients (85% vs 59%, P<0.001).

Conclusion R1 resections portend poorer survival than R0 but do not negate the benefit of surgery when additional therapy is given. NAT was associated with improved R0 rates and improved survival for stage III positive-margin patients.

  • pancreatic cancer
  • surgical margins
  • NCDB

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Key questions

What is already known about this subject?

  • It is known that R1 resections portend worse prognosis than R0 resections in pancreas cancer, but the magnitude of this detriment and the effect of neoadjuvant or adjuvant therapy on margin positive resections is unknown.

What does this study add?

  • In an aggregate dataset of 44 852 patients, we found that median survival after R1 resection was 14.3 months compared with 19.7 months for R0 resection. Median survival for margin positive patients was further improved by neoadjuvant therapy (17.6 months) or adjuvant therapy (16.8 months). Furthermore, R0 rates were improved after neoadjuvant therapy.

How might this impact on clinical practice?

  • These data may encourage resection in more patients who receive appropriate neoadjuvant treatment even if the risk of R1 resection is significant.


Pancreatic cancer is a deadly disease; even patients who present with localised tumours have a median survival (MS) of fewer than 3 years.1–8 Most patients present with metastatic disease or advanced tumours that abut or encase the vasculature and thus are difficult to resect with negative margins. Both prospective8 9 and retrospective series10–16 have found that survival is reduced if surgical margins are positive, even microscopically. There is uncertainty over the acceptable rate of margin-positive resections with older data suggesting that a margin-positive resection portends survival similar to no resection at all.17 Only more recently have randomised clinical trials included patients with R1 resections in order to examine the most appropriate care in the adjuvant setting.3 4 8 18

Whether a margin-positive resection is equivalent to best non-surgical therapy has not been examined thoroughly in the era of neoadjuvant therapy (NAT). NAT has theoretical advantages in this setting, including a reduced rate of positive margins, a potential to downstage the primary tumour, and the ability to address microscopic distant disease earlier than with adjuvant therapy (AT).19–22 Additionally, given the propensity of pancreatic cancer to progress distantly, patients who progress through NAT may be spared the morbidity of a surgery that would not have resulted in a survival benefit.19–22 A common clinical question is how to proceed with patients who do not progress through NAT but remain at risk of positive margins based on imaging evaluation after NAT.

In order to better quantify the effects of surgical margins on survival, we sought to determine the benefit of surgical resection in patients who underwent surgery with positive and negative margins to those who underwent non-surgical treatment (chemotherapy or chemoradiotherapy (CRT)). We further sought to quantify the effect of a margin-positive resection on survival and whether the presence and timing of additional therapy impacted that survival. To our knowledge, no properly powered study has examined the role of margin status, timing of therapy and benefit of surgical resection.

Methods and materials

Patient data were obtained from the National Cancer Database (NCDB), a joint programme of the Commission on Cancer and the American Cancer Society, which includes data from approximately 1500 hospitals and clinics in the USA and its territories. This database captures nearly 70% of new cancer diagnoses made in the USA.23 All NCDB data are deidentified and therefore exempt from review by our institutional review board.

The initial query included all patients with pancreatic cancer diagnosed between the years of 2004 and 2013, which yielded 309 709 patients (online supplementary figure 1). Patients with clinical stage II or III disease were selected for analysis (44 852). Stage refers to clinical stage throughout the analysis unless otherwise specified. Patients were first separated into groups by treatment strategy: surgery alone (Surg), NAT followed by surgery, AT following surgery and no surgery (chemotherapy or CRT alone) to ascertain whether there were differences between the treatment groups that might explain potential survival differences. The primary outcome variable was survival based on surgical resection and margin status. Survival was compared between patients who underwent no surgery, surgery with negative margins (R0), surgery with microscopically positive margins (R1) and surgery with macroscopically positive margins (R2) using univariate (UVA) Cox proportional hazards modelling. A P value of 0.05 was required for significance.

Supplementary file 1

Patients were separated into cohorts by treatment status, timing of treatment and surgical margin status. Treatment cohorts included: no surgery (NoSurg), NAT followed by surgical resection with either positive (NAT/Pos) or negative (NAT/Neg) margins, upfront surgery followed by treatment with either positive (AT/Pos) or negative margins (AT/Neg) and surgery alone with either positive (Surg/Pos) or negative margins (Surg/Neg). Univariate and multivariate (MVA) Cox proportional hazards modelling were done. Factors with a P value of 0.2 or lower were included in the MVA. A P value of 0.05 or lower was required for significance on MVA.

Patients were further broken down by clinical stages (IIA, IIB and III), and UVA Cox proportional hazards modelling was done based on resection and margin status. The margin-negative resection rates were also obtained by clinical stage. Patients who underwent surgery and had positive margins were then isolated in order to determine the effect of the presence and timing of additional therapies via UVA and MVA. Patients were separated into the following cohorts: NAT, AT and Surg (no additional therapy). Finally, patients who underwent surgical resection were isolated to identify predictors of positive margins via UVA and MVA.

All analyses were performed using the STATA V.14.0 statistical package.


After all exclusions had been made, 44 852 patients were available for analysis. Patient characteristics were examined for Surg, NAT followed by surgery, surgery followed by AT and NoSurg patients (table 1). Patients who did not undergo surgery tended to have bigger tumours (18% greater than 5 cm vs 10%–13% P<0.001), be clinically node-positive (42% vs 33%–36%, P<0.001) and be clinical stage III (59% vs 6%–23%, P<0.001) than patients who did. Patients treated neoadjuvantly tended to have tumours located in the pancreatic head (77% vs 63%–74%, P<0.001) and be clinical stage III compared with patients treated with surgery upfront (23% vs 6%–8%, P<0.001). They were equally likely, however, to be stage III pathologically (5% vs 4%–5%, P<0.001) and were less likely to have positive lymph nodes at surgery (46% vs 66%–73%, P<0.001), though clinical node positivity did not vary by more than 3% between the groups. Negative margins were more likely in NAT (88% vs 81%, P<0.001) than patients treated with surgery upfront. Patients treated with NAT were more likely to be treated with CRT (69% vs 46%–50%, P<0.001) and multiagent chemotherapy (58% vs 31%–44%, P<0.001) compared with other groups. Thirty-day (2% NAT vs 3.6% AT+Surg, P<0.001) and 90-day (6% NAT vs 7% AT+Surg, P<0.001) mortality were not significantly different between NAT and surgery upfront patients (AT+Surg).

Table 1

Patient characteristics (n=44 852)

Patients who underwent R0 (MS 19.7 months, P<0.001) or R1 (MS 14.3 months, P<0.001) resections experienced a survival benefit compared with NoSurg patients (MS 10.3 months) (figure 1). Patients who underwent R2 resections experienced poorer survival (MS 9.5 months, P=0.07) that was not significantly different than that of NoSurg patients.

Figure 1

Overall survival by margin status and surgical resection.

When survival was compared by surgical resection status, margin status and presence/timing of additional therapy, all groups experienced improved survival outcomes over NoSurg (MS 10.3 months), except for the Surg/Pos patients (MS 6.8 months, P<0.001) (see table 2, online supplementary figure 2). This finding remained significant on MVA (see table 3). The highest survival was achieved by NAT/Neg patients compared with all other cohorts (table 2). There was no difference in survival between NAT/Pos and AT/Pos patients, but both groups experienced improved survival compared with Surg/Pos patients (tables 2 and 3). Both NAT/Neg and AT/Neg patients achieved superior survival compared with the Surg/Neg patients (see table 2).

Supplementary file 2

Table 2

Median and 5-year overall survival by treatment, timing of treatment and stage

Table 3

Multivariate analysis for survival in all patients/margin positive patients

When positive-margin patients were isolated as a group on MVA, AT was equal to NAT (HR 1.11, P=0.17) (table 3). Treatment with CRT (HR 0.78, P<0.001) but not multiagent chemotherapy (HR 0.93, P=0.199) was associated with a decreased risk of death. When broken down by clinical stage, NAT/Pos and AT/Pos yielded equivalent survival for stage IIA and IIB patients (see table 2). For stage III patients, NAT/Pos patients experienced improved survival compared with AT/Pos and Surg/Neg patients and similar to AT/Neg patients. When NAT was given, the margin-positive rate decreased by approximately 5% for stage II patients and 26% for stage III patients (table 2).

Finally, predictors of positive margins included Charlson score, tumour size, clinical stage, pathological stage and timing of therapy. Tumours greater than 2 cm (OR 1.59, P<0.001), greater than 5 cm (OR 2.03, P<0.001), grade 3 tumours (OR 1.19, P=0.03), clinical stage III (OR 1.65, P<0.001), pathological stage III (OR 2.46, P<0.001) and stage IV (OR 1.86, P=0.009) were all associated with positive margins. Upfront surgery was associated with increased risk of positive margins compared with NAT (OR 1.69, P<0.001).


In patients with stage II and III pancreas cancer, surgical resection was associated with a survival benefit compared with patients who did not undergo surgery. This benefit was maximised in patients able to undergo R0 resection, though patients who underwent an R1 resection still experienced a statistically and clinically significant survival benefit compared with patients who did not undergo surgery, adjusting for available cofactors. While we could not control for every difference that may exist between the surgical and non-surgical patients, the observation of improved outcomes even for R1 resection represents a possible imbalance in the current practice patterns. There was no survival benefit after an R2 resection. The timing of additional therapy also affected the relevance of marginal status in patients who underwent surgical resection. Neoadjuvant treatment was associated with improved survival compared with AT if margins were negative, and there was no difference in survival if margins were positive. Surgical resection alone with positive margins was no better than no surgery. For stage III patients, NAT/Pos was associated with improved survival compared with AT/Pos with improved margin-negative resection rate (85% vs 59%).

In this study, patients who underwent an R1 resection were half as likely to be alive at 5 years as patients who underwent R0 resection, though they were twice as likely to be alive at 5 years when compared with patients who did not undergo surgery. Perioperative deaths were not censored from this analysis. Thus, it does appear that surgical resection is associated with a survival benefit compared with no surgery, and an R1 resection, while detrimental, does not negate that benefit. R2 resections, however, offer no survival benefit. This finding was confirmed when patients were stratified by stage. This is concordant with a previous multicentre randomised study in resectable pancreatic cancer that compared definitive chemoradiotherapy to surgical resection alone: MS was almost 1 year longer for the surgical group, with a 3-year survival rate gain of 20% compared with definitive chemoradiotherapy.24 In terms of surgical margins, these data are difficult to compare with other published series, as (1) these series do not compare their survival results to non-surgical results,1 8 9 11–18 25 (2) these series are mostly in the setting of AT1 8 9 11–18 25 and (3) R2 resections are often excluded1 8 18 25 (table 4). No matter the initial extent of disease, R0 resections trend towards improved survival in the majority of retrospective series and in the most recent prospective adjuvant study.8 9 11–17 25 Some aggregated prospective data, however, show no effect of margin status on survival.3 4 It is interesting that NAT was associated with a survival benefit in margin-negative patients but was no different than AT for margin-positive patients. This finding may be secondary to attrition in the NAT group, as a certain percentage of patients may not proceed to surgery due to disease progression, decompensation or other reasons. In patients with negative margins, this may be the driver of the perceived survival benefit. In the positive-margin patients, survival may have been affected by margin status such that any perceived benefit for the NAT group was offset by the survival detriment of a margin-positive resection.

Table 4

Selected publications about the value of neoadjuvant or adjuvant therapy in relation to margin status

The exception to this may be in patients at highest risk for positive margins (stage III). In these patients, NAT may allow for more successful surgeries. In our series, the overall cohort experienced an improved R0 rate with NAT (88% vs 81%, P<0.001), and stage III patients experienced a significant improvement (85% vs 59%, P<0.001). These data are concordant with other series demonstrating significantly reduced margin-positive resection rates with NAT (12.3% vs 19%–51%).4 17 18 26–28 In stage III patients, NAT/Pos was associated with a survival benefit compared with AT/Pos and equivalent survival to AT/Neg. These data suggest that NAT should be considered for all patients with borderline or locally advanced disease, which is concordant with current National Comprehensive Cancer Network  (NCCN) guidelines and the subject of two ongoing clinical trials.29 30 For resectable disease, NAT should be considered based on the improvement in survival seen in margin-negative patients as well as the improved margin-negative rate.

Unfortunately, in the entire cohort of patients treated with CT or CRT upfront, only 11% of patients underwent resection, which is consistent with available literature including the recently reported results from LAP-07, where only 4% of patients with locally advanced pancreas cancer (LAPC) (stage III) underwent surgery after treatment.31 Some series using NAT in borderline and LAPC report higher resection rates ranging from 31% to 67%, with R0 resection rates ranging from 86% to 100%.26 28 32 Although the number of patients able to undergo resection was fewer in this study, the percentage of R0 resections was consistent (88%). MS for patients treated with NAT who underwent resection in these series ranged from 21.7 to 32 months, also consistent with our data (23 months with R0 resection). Ferrone et al 33, reported on 40 patients with LAPC treated with FOLFIRINOX and radiotherapy followed by resection. Although nearly half (19) of patients were deemed unresectable by imaging after NAT, the R0 resection rate was 90%.

The major weakness of this study is that it is difficult to compare surgical versus non-surgical patients as we do not have access to detailed reasons why surgery was or was not part of the planned course of treatment. This would be especially helpful in stage IIA-B ‘resectable’ patients who did not undergo surgery and for stage III ‘borderline/locally advanced’ patients who did, as these patients may be representative of extenuating circumstances that may create bias. Though guidelines for resectability exist, significant heterogeneity likely remains. Even with heterogeneity (which ideally would decrease selection bias), it is possible that the healthiest patients were consistently more likely to receive the most aggressive treatments (ie, multiagent chemotherapy, CRT and surgery). Thus, the perceived survival benefit in these patients could have been enhanced by a disproportionate inclusion of patients who were likely to live longer regardless of treatment. Also, patients who began treatment with localised disease may have developed distant or otherwise unresectable disease during the course of treatment and thus may not have been candidates for aggressive local treatment. Patients in the no surgery group may also have had findings suspicious for metastatic disease at presentation or were otherwise deemed medically ineligible for surgery (ie, elevated CA-19–9 and severe weight loss). The presence of these patients in high numbers could certainly affect the perceived survival benefit. Finally, the definition of an R1 resection can vary between studies, and more strict standardisation of pathology processing can increase the margin positivity rate substantially.34 It is further suggested that survival is affected by distance of tumour to the margin even over the range of 0–1.5 mm, which is not standardly reported.35

Though these weaknesses are significant and a source of potential selection bias, there are also important strengths in this study. Over 44 000 patients were examined, which lends significant power. Additionally, these patients were treated by physicians from different institutional settings whose practice patterns and expertise likely varied, giving an accurate national picture of practice patterns and the current state of the field, which adds to the external validity of these results. There will likely never be a randomised study addressing the value of surgery in these high-risk patients, so large retrospective studies can add value by providing hypothesis-generating data to guide future prospective studies.


An overall survival benefit to surgical resection, even in microscopically positive-margin patients, is demonstrated in this large aggregated dataset for patients with clinical stage IIA–III pancreatic cancer. Patients who underwent R1 resections were twice as likely to be alive at 5 years compared with patients who did not undergo resection, though only half as likely as patients who underwent R0 resections. The timing of additional therapies affects the significance of margin status on survival. NAT was associated with improved R0 resection rate and improved survival in the R0 setting. NAT in R1 resections was equivalent to AT for stage IIA-B patients but superior for stage III patients. Thus, NAT should be favoured in borderline and LAPC. Given the improved outcomes seen in patients with an R1 resection compared with patients who do not undergo surgery, these data suggest surgical resection should be heavily considered even if the significant possibility of an R1 resection exists .


The authors would like to acknowledge Michelle Denney for her assistance in copyediting and manuscript preparation.


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  • Contributors Concept and design: SL and AT. Statistical analysis: SL and AT. Manuscript writing, editing and final version: all authors.

  • Funding This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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