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Correction: Key differences between 13 KRAS mutation detection technologies and their relevance for clinical practice

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Sherwood JL, Brown H, Rettino A, et al. Key differences between 13 KRAS mutation detection technologies and their relevance for clinical practice. ESMO Open 2017;2:e000235.  10.1136/esmoopen-2017-000235

Tables 3A and 3B have been amended to reflect the error in the use of reagents by the third-party laboratory (IMGM, Munich) for the ddPCR Q61H assay (see appendix). The therascreen® KRAS RGQ PCR Kit Q61 assay does not test for Q61H; the PrimePCR™ ddPCR™ Mutation Assay was not performed for Q61H due to an error at the participating laboratory. This was identified after publication, as referred to in the Letter to the Editor (http://dx.doi.org/10.1136/esmoopen-2017-000294). A new abbreviation “NP” = “not performed” has been added to explain that no ddPCR assay for codon Q61 was performed.

In the abstract, the corrected sentence now reflects a lower number of total data points (718 instead of 728) because of the removal of 5 data points for ddPCR p.Q61H mutation results for 100 mutant copies input and 5 data points for 50 mutant copies input, respectively. Overall 406/718 data points across all 13 technologies were identified correctly. The digital PCR assay (KRAS PrimePCR™ ddPCR™, Bio-Rad Laboratories) identified 70% (100 copies) and 65% (50 copies) of samples correctly.

The ddPCR results section is corrected to reflect that for codon Q61H, the incorrect PrimePCR™ KRAS mutation assay had been used (p.Q61H c.183A>C instead of p.Q61H c.183A>T assay): The PrimePCR ddPCR KRAS Mutation Assays were able to identify codon 12 and 13 mutations down to 1% with the 100 copy input. However, across both admixture and wild-type control samples the assay identified the incorrect mutation in nine different mutation/allele frequency combinations (see table 3). When performing the p.Q61H assay, a mistake was made and the incorrect reagent detection of c.183A>C instead of c.183A>T, was used. Therefore, table 3 and figure 1 reflect data only for codons 12 and 13.

Table 3

KRAS mutation detection success by codon, concentration and technology for (A.) 100 mutant copies input, (B.) 50 mutant copies input and (C.) wild-type only.

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